Since 2014
PREPARATION OF CHEMICAL AND PHYSICAL CONJUGATES OF SELF-ASSEMBLING NANOPARTICLES WITH CELL-PENETRATING PEPTIDE AND DOXORUBICIN
CBU International Conference on Innovation in Science and Education
View Archive Info| Field | Value | |
| Title |
PREPARATION OF CHEMICAL AND PHYSICAL CONJUGATES OF SELF-ASSEMBLING NANOPARTICLES WITH CELL-PENETRATING PEPTIDE AND DOXORUBICIN
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| Creator |
Shagyrova, Zhadyra Sagykyzy; National Center for Biotechnology
Zhienbay, Yerzhan Yersaiynuly; Nazarbayev University Research and Innovation System (NURIS), Nazarbayev University Voikov, Mikhail Seregeyevich; Nazarbayev University Research and Innovation System (NURIS), Nazarbayev University Shustov, Alexander Vyacheslavovich; National Center for Biotechnology |
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| Subject |
virus-like particles, drug carrier, targeting, conjugate, transportan, Doxorubicin
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| Description |
Abstract: Nano-sized carriers can help to reduce toxicity and improve clinical efficacy of drugs. Virus-like particles (VLPs) are biocompatible and biodegradable self-assembling nanoparticles, which show great promise as carriers for substances for targeted delivery and controlled release. Either chemical conjugation of physical incorporation without formation of covalent bonds is possible to load substances of interest into VLPs.Objectives: To produce VLPs from recombinant viral capsid protein (HBcAg) and test feasibility of methods of formation of chemical and physical conjugates of VLPs with substances of pharmacological interest.Methods: Virus-like particles composed from recombinant hepatitis B core antigen (HBcAg) were produced by recombinant expression in E.coli and purified by successive centrifugation through sucrose gradients. Peptide transportan 10 was synthesized and used for carbodiimide (EDC)-mediated conjugation to VLPs. Doxorubicin (DOX) was loaded into the nucleic acid-containing VLPs to form physical conjugate.Results: VLPs with chemically attached moieties of cell-penetrating peptide transportan 10 were produced. The conjugate was examined in SDS-PAGE to confirm presence of conjugation products. Conjugation efficiency (molar ration peptide/protein in the conjugate) reaches 0.5:1 (i.e. 50% of protein chains have one attached peptide moiety). The nucleic acid-containing VLPs can be loaded with the DOX forming stable non-covalent physical conjugate.Conclusion: Recombinantly expressed VLPs allow easy attaching of small molecules making them a convenient platform to develop drug carriers.
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| Publisher |
Central Bohemia University, o.p.s.
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| Contributor |
—
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| Date |
2015-09-19
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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| Format |
application/pdf
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| Identifier |
http://ojs.journals.cz/index.php/CBUConference2013/article/view/630
10.12955/cbup.v3.630 |
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| Source |
CBU International Conference Proceedings; Vol 3 (2015): CBU International Conference Proceedings 2015; 405-411
1805-9961 1805-997X |
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| Language |
eng
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| Relation |
http://ojs.journals.cz/index.php/CBUConference2013/article/view/630/584
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| Rights |
Copyright (c) 2015 Zhadyra Sagykyzy Shagyrova, Yerzhan Yersaiynuly Zhienbay, Mikhail Seregeyevich Voikov, Alexander Vyacheslavovich Shustov
https://creativecommons.org/licenses/by/3.0/ |
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