Record Details

Optimized Conditions for the Production of Hydroxamic Fatty Acids from Coconut Oil by a Lipase from Rhizopus delemar

BANWA Archives (2004-2013)

View Archive Info
 
 
Field Value
 
Title Optimized Conditions for the Production of Hydroxamic Fatty Acids from Coconut Oil by a Lipase from Rhizopus delemar
 
Creator Fundador, Noreen G.V.
Castillo, Eugenia M.
 
Description Alipase from Rhizopus delemar was used as a biocatalyst for the synthesis of hydroxamic fatty acids (HFA) by reacting hydroxylamine with coconut oil, methyl laurate and lauric acid. The effects of various reaction parameters such as amount of hydroxylamine-HCl, incubation time, temperature, and amount of enzyme were evaluated to achieve high product yield.The reaction products were monitored by Thin Layer Chromatography using hexane: diethyl ether: acetic acid (20:80:1 v/v/v) as solvent system. Identification of the products was done by Gas-Liquid Chromatography. Analysis revealed a mixture of HFA produced using coconut oil as substrate: 30.33% myristylhydroxamic acid (MHA), 24.17% olelyhydroxamic acid (OHA) and 45.50% laurylhydroxamic acid (LHA). On the other hand, methyl laurate and lauric acid yielded only LHA.Purification of the product was done by crystallization in hexane at 14 °C. Identification of the functional groups present was determined by Infrared (IR) spectroscopy.
 
Publisher University of the Philippines Mindanao
 
Date 2015-09-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://ojs.upmin.edu.ph/index.php/banwa-archives/article/view/32
 
Source BANWA Archives (2004-2013); Vol 4 No 2 (2007); 33-44
1656-3719
 
Language eng
 
Relation http://ojs.upmin.edu.ph/index.php/banwa-archives/article/view/32/37
 
Rights Copyright (c) 2015 Noreen G.V. Fundador, Eugenia M. Castillo
http://creativecommons.org/licenses/by-nc-nd/4.0