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Tibetan hulless barley dehydrin, dhn4, cloning and transforming into tobacco

Journal of Agriculture and Environment for International Development

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Title Tibetan hulless barley dehydrin, dhn4, cloning and transforming into tobacco
 
Creator Wang, Jianhui
Chen, Kelin
Li, Hogwen
He, Jian
Guan, Bin
Du, Junbo
Liu, Jianjun
 
Description A dehydrin,dhn4, cDNA fragment has been obtained via RT-PCR from Tibetan hulless barley(Hordeum vulgereL. var. nudum Hook. f.). It indicated that dhn4encoded a YSK2 type dehydrin (DHN4). One Y segment (VDEYGNP), one S segment (SGSSSSSSS) and two K segments (RKKGIKEKIKEKLPG and EKKGIMDKIKEKLPG) were identified in the deduced amino acid sequence of dhn4.
The secondary structure of DHN4 protein predicated with software Anthepro 5.0 is prone to ?-helix, and the tertiary structure predicated by SWISS-PORT indicated intrinsically unstructured. The coding region of the dhn4 cloned into pBI121 binary vector with the 35S promoter was transformed into the Agrobacterium tumefaciens strain DHA105.
The Agrobacterium mediation was transformed dhn4 into the leaf disc of tobacco and then the tobacco plantlets with kanamycin resistant were regenerated using callus induction mediums supplemented with kanamycin and carbencillin. The regenerated plants were transferred into plots with peat moss and grown in the greenhouse. The inserting dhn4 of regenerated plants were identified separately by PCR, PCR southern blot and DNA sequencing using the gnomic DNA.
 
Publisher Italian Agency for Development Cooperation
 
Contributor —
 
Date 2011-11-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://www.iao.florence.it/ojs/index.php/JAEID/article/view/30
10.12895/jaeid.20093.30
 
Source Journal of Agriculture and Environment for International Development; Vol 103, No 3 (2009); 173-184
Journal of Agriculture and Environment for International Development (JAEID); Vol 103, No 3 (2009); 173-184
2240-2802
1590-7198
 
Language eng
 
Relation http://www.iao.florence.it/ojs/index.php/JAEID/article/view/30/34
 
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