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Effect of Hyperbaric Oxygenation on the Level of Free Fatty Acids in Experimental Spinal Cord Injury in Rabbits

Croatian International Relations Review

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Title Effect of Hyperbaric Oxygenation on the Level of Free Fatty Acids in Experimental Spinal Cord Injury in Rabbits
Utjecaj hiperbarične oksigenacije na razinu slobodnih masnih kiselina u eksperimentalnome modelu kontuzije leđne moždine kunića
 
Creator Pantović, Radojka
Draganić, Pero
Eraković, Vesna
Blagović, Branka
Milin, Čedomila
Simonić, Ante
 
Subject free fatty acids; hyperbaric oxygenation; spinal cord injury; rabbit
 
Description The aim of this study was to investigate the influence of hyperbaric oxygenation treatment (HBOT), a potent inhibitor of tissue hypoxia, on the motor activity and the spinal cord tissue concentration of free palmitic (C16:0), stearic (C18:0), oleic (C18:1n-9), arachidon (C20:4n-6) and docosahexaenoic (C22:6n-3) acids, in rabbits with spinal cord injury (SCI). SCI resulted in paraplegia and accumulation of all analyzed free fatty acids (FFAs) in the spinal cord after neurotrauma. HBOT (222.915 kPa pressure of 99.5 % oxygen for 45 min), applied immediately after injury and followed by 8 consecutive daily treatments, improved motor impairment and completely prevented the SCI-induced increase in FFAs. These data suggest that a perturbation of the membrane lipid metabolism may contribute to the functional deficit associated with SCI, and HBOT may protect the injured spinal cord tissue, at least in part, by limiting these posttraumatic membrane lipid changes.
Cilj ovoga rada bio je istražiti utjecaj hiperbarične oksigenacije, potentnoga sredstva korekcije hipoksije, na motoričku aktivnost kunića s povredom leđne moždine i koncentraciju slobodnih masnih kiselina (FFA, free fatty acid) u uzorcima tkiva povrijeđene moždine, i to palmitinske (C16:0), stearinske (C18:0), oleinske (C18:1n-9), arahidonske (C20:4n-6) i dokozaheksaenske (C22:6n-3). Rezultat eksperimentalne povrede leđne moždine je teški motorički deficit u kunića, ali i akumulacija svih analiziranih FFA. Hiperbarična oksigenacija (HBOT), terapija udisanjem 100 % kisika u uvjetima povišenog tlaka (222.915 kPa kroz 45 minuta) neposredno nakon povrede te još 8 uzastopnih dana, popravila je motoričko oštećenje i u potpunosti zaustavila porast koncentracije FFA uzrokovan povredom. Ovi podaci ukazuju da poremećaj u metabolizmu membranskih lipida može doprinijeti funkcionalnome deficitu, koji je posljedica povrede leđne moždine, kao i da HBOT može zaštititi povrijeđeno tkivo, ograničavajući posttraumatske promjene membranskih lipida.
 
Publisher Croatian Chemical Society
 
Date 2005-09-15
 
Type text
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://hrcak.srce.hr/57
http://hrcak.srce.hr/file/57
 
Source Croatica Chemica Acta; Vol.78 No.3; ISSN 0011-1643 (Print); ISSN 1334-417X (Online)
 
Language en
 
Rights info:eu-repo/semantics/openAccess
Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.