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Effects of Aspirin and Indomethacin on Galectin-3

Croatian International Relations Review

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Title Effects of Aspirin and Indomethacin on Galectin-3
Utjecaj aspirina i indometacina na galektin-3
 
Creator Dabelić, Sanja
Flögel, Mirna
Dumić, Jerka
 
Subject aspirin; galectin-3; indomethacin; LGALS3
 
Description Galectin-3, a β-galactoside binding lectin, acts as a strong pro inflammatory signal. Many immunomodulatory drugs affect signaling pathways that comprise transcription factors involved in regulation of galectin-3 gene (LGALS3) expression. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs aspirin and indomethacin on the expression of galectin-3, both on mRNA and protein levels. The human monocytic cell line THP-1 was exposed to various therapeutic concentrations of aspirin and indomethacin for 72 hours. Relative RT-PCR method and the GeneScan analysis software were used for assessing the galectin-3 mRNA level and chemiluminescent-western blot analysis was used for measuring the galectin-3 level. The results showed that galectin-3 is a new target molecule of NSAIDs, which cause reduction of both gene and protein expression of galectin-3, but the intensity and time-course of the changes strongly depend on the kind and concentration of the drugs.
Galektin-3, lektin koji veže β-galaktozidne jedinice, djeluje kao jak proinflamatorni signal. Mnoge imunomodulatorne tvari utječu na signalne putove i transkripcijske faktore uključene i u regulaciju ekspresije gena za galektin-3 (LGALS3). Cilj je ovoga rada bio ispitati učinke nesteroidnih tvari s imunomodulatornim djelovanjem – aspirina i indometacina – na ekspresiju galektina-3, kako na razini mRNA tako i na razini proteina. Stanice humane stanične linije THP-1 izložene su različitim terapijskim koncentracijama aspirina i indometacina tijekom 72 sata. Količina mRNA za galektin-3 odre|ena je primjenom metode RT-PCR i analize programom GeneScan, a razina galektina-3 kemiluminescentnom western-blot analizom. Rezultati su pokazali da galektin-3 predstavlja novu ciljnu molekulu djelovanja nesteroidnih imunomodulatornih tvari, koje uzrokuju smanjenje ekspresije gena za galektin-3 i samoga proteina, premda jakost promjena i vrijeme njihove pojave jako ovise o vrsti i koncentraciji primijenjene tvari.
 
Publisher Croatian Chemical Society
 
Date 2005-09-15
 
Type text
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://hrcak.srce.hr/54
http://hrcak.srce.hr/file/54
 
Source Croatica Chemica Acta; Vol.78 No.3; ISSN 0011-1643 (Print); ISSN 1334-417X (Online)
 
Language en
 
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Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.