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Concentration-dependent Effects of Peroxovanadium Compound bpV(phen) on PC12 Cell Survival

Croatian International Relations Review

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Title Concentration-dependent Effects of Peroxovanadium Compound bpV(phen) on PC12 Cell Survival
Utjecaj različitih koncentracija peroksovanadijevog spoja bpV(phen) na preživljavanje PC12 stanica
 
Creator Rumora, Lada
Barišić, Karmela
Petrik, József
Žanić Grubišić, Tihana
 
Subject peroxovanadium compounds; MAPK; caspase; apoptosis; PC12 cells
 
Description Peroxovanadium compounds are potent insulinomimetic agents and protein tyrosine phosphatase inhibitors. In this study, the potential toxicity of peroxovanadium complex bpV(phen) on rat pheochromocytoma PC12 cells was examined, and the mechanism by which this compound influences cell survival and/or death was explored. BpV(phen) exerted a bimodal effect on PC12 cells: survival enhancing effect at lower and death-inducing effect at higher micromolar concentrations. 1 and 3 μmol dm–3 bpV(phen) intensely induced ERK activation. In contrast, 10 and 100 μmol dm–3 bp (phen) stimulated strong and sustained JNK and p38 MAPK activation as well as caspase-3 activation that preceded bpV(phen)-induced apoptotic cell death. It is suggested that bpV(phen) might exert its action on PC12 cell survival by modulation of MAPKs and caspase-3 activation.
Spojevi peroksovanadija snažni su inzulinomimetički agensi i inhibitori tirozinskih fosfataza. U ovom istraživanju ispitivana je moguća toksičnost peroksovanadijevoga kompleksa bpV(phen) na PC12 stanicama feokromocitoma štakora te način na koji ovaj spoj utječe na preživljavanje i/ili umiranje stanica. BpV(phen) dvojako je djelovao na PC12 stanice: primijenjen u nižim mikromolarnim koncentracijama poticao je preživljavanje stanica, dok su više mikromolarne koncentracije poticale umiranje stanica. 1 i 3 μmol dm–3 bpV(phen) snažno je inducirao aktivaciju ERK kinaza. Suprotno tome, 10 i 100 μmol dm–3 bpV(phen) stimulirao je snažnu i dugotrajnu aktivaciju JNK i p38 MAPK kinaza te aktivaciju kaspaze-3, što je prethodilo umiranju stanica procesom apoptoze. Pretpostavlja se da bpV(phen) djeluje na preživljavanje PC12 stanica na način da modulira aktivaciju MAPK kinaza i kaspaze-3.
 
Publisher Croatian Chemical Society
 
Date 2005-09-15
 
Type text
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://hrcak.srce.hr/52
http://hrcak.srce.hr/file/52
 
Source Croatica Chemica Acta; Vol.78 No.3; ISSN 0011-1643 (Print); ISSN 1334-417X (Online)
 
Language en
 
Rights info:eu-repo/semantics/openAccess
Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.