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Local Variability in the Mechanics of Titin’s Tandem Ig Segments

Croatian International Relations Review

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Title Local Variability in the Mechanics of Titin’s Tandem Ig Segments
Lokalna promjenljivost (nestalnost) mehaničkih svojstava tandemskih Ig/segmenata titina
 
Creator Grama, László
Nagy, Attila
Scholl, Clara
Huber, Tamas
Kellermayer, Miklós S. Z.
 
Subject titin; immunoglobulin domain; elasticity; single-molecule force spectroscopy; unfolding
 
Description The functionally elastic, I-band part of the myofibrillar protein titin (connectin) contains differentially expressed arrays of serially linked immunoglobulin (Ig)-like domains, the length and composition of which vary among the titin isoforms. The biological rationale of the differential expression as well as the contribution of the Ig domain mechanical characteristics to the overall mechanical behavior of titin are not exactly known. The paper briefly reviews the relevant works that have addressed the Ig-domain mechanics problems and presents the authors’ experimental approach to studying the mechanical behavior of Ig domains. The mechanics of an eight domain segment from the differentially expressed tandem Ig region of titin (I55-62) was studied with an atomic force microscope specially used for stretching single molecules, and the results were compared to known mechanical properties of other domains and segments.
Funkcionalno elastična I-vrpca miofibrilarnoga proteina titina (konektina) sadrži razlicito izražene vrste serijski povezanih domena sličnih imunoglobulinu (Ig) čija se duljina i sastav razlikuju u pojedinim titinskim izoformama. Biološki razlozi diferencijalne ekspresije kao i doprinos mehaničkih svojstava domena koje su slične Ig ponašanju titina nisu u cjelosti poznati. U ovom su članku pregledno prikazani dosadašnji relevantni radovi koji se bave problemima mehaničkih svojstava Ig-domena te autorski eksperimentalni pristupi u istraživanju toga problema. U radu su također prikazani rezultati istraživanja mehaničkih svojstava diferencijalno eksprimiranoga tandemskoga Ig-područja titina (155-62), koji se sastoji od osam domena, pomoću mikroskopa atomske snage razlučivanja specijaliziranoga za rastezanje pojedinačne molekule. Ovim postupkom utvrđena mehanička svojstva ispitivanoga dijela titinske molekule uspoređena su s poznatim mehaničkim svojstvima drugih domena i segmenata.
 
Publisher Croatian Chemical Society
 
Date 2005-09-15
 
Type text
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://hrcak.srce.hr/50
http://hrcak.srce.hr/file/50
 
Source Croatica Chemica Acta; Vol.78 No.3; ISSN 0011-1643 (Print); ISSN 1334-417X (Online)
 
Language en
 
Rights info:eu-repo/semantics/openAccess
Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.