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Does Long-term Exercise Modulate Oxidant/Antioxidant Status in Humans? Comparison between Lipid Peroxidation and Catalase Activity in Fresh and Stored Samples

Croatian International Relations Review

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Title Does Long-term Exercise Modulate Oxidant/Antioxidant Status in Humans? Comparison between Lipid Peroxidation and Catalase Activity in Fresh and Stored Samples
Modulira li dugotrajno vježbanje oksidacijski/antioksidacijski status ljudi? Usporedba između lipidne peroksidacije i aktivnosti katalaze u svježoj i pohranjenoj plazmi
 
Creator Šverko, Ana
Sobočanec, Sandra
Balog, Tihomir
Kušić, Borka
Šverko, Višnja
Marotti, Tanja
 
Subject lipid peroxidation; exercise; catalase; oxidant; antioxidant
 
Description Physical activity may have beneficial effects on the balance between oxidant stress and the defense system. The purpose of this study was to evaluate the influence of exercise on oxidant/antioxidant status in both sexes during autumn and winter periods. Since all women were tested in a low estrogen phase, the possibility to test the same parameters in plasma stored at low temperature would be of great importance. Oxidant changes in plasma were determined by measuring the concentration of thiobarbituric acid reactive substances (TBARS). Antioxidant capacity was measured by catalas (CAT) activity in lysate of erythrocytes. The results showed significantly higher TBARS concentrations in untrained men compared to women. Antioxidant capacity was of the same value in both sexes. Exercise did not change the oxidant/antioxidant status of either sex. The results indicate that women were more protected from oxidant processes than men. This finding points to the role of lipid peroxidation in higher incidence of myocardial infarction in men during winter. Significant changes in the oxidant/antioxidant status in stored plasma allowed greater reliability of TBARS and CAT measurements only in fresh samples.
Tjelesna aktivnost može imati korisne učinke na ravnotežu između oksidativnoga stresa i obrambenoga sustava. Svrha ovog rada je bila procijeniti utjecaj vježbanja na oksidacijski/antioksidacijski status u oba spola tijekom jeseni i zime. Budući da su žene bile istraživane u niskoj estrogenskoj fazi od velike je važnosti moguć nost ispitivanja nekih pokazatelja u plazmi pohranjenoj na niskim temperaturama. Oksidacijske promjene u plazmi određene su mjerenjem koncentracije tiobarbiturno reaktivnih supstanci (TBARS). Antioksidativna sposobnost mjerena je aktivnošću katalaze (CAT). Rezultati su pokazali statistički značajno visoku koncentraciju TBARSa u netreniranih muškaraca u odnosu na žene. Nasuprot tome, antioksidacijska sposobnost bila je iste razine u oba spola. Vježbanje nije promjenilo oksidacijski/antioksidacijski status u oba spola. Rezulti pokazuju da su žene zaštićenije od oksidacijskih procesa od muškaraca. Taj nalaz ukazuje na ulogu lipidne peroksidacije u većoj pojavnosti srčanoga infarkta u muškaraca. Značajne promjene u oksidacijskom/antioksidacijskom statusu u pohranjenoj plazmi dopuštaju veću pouzdanost mjerenja TBARSa i CATa samo u svježoj plazmi.
 
Publisher Croatian Chemical Society
 
Date 2005-09-15
 
Type text
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://hrcak.srce.hr/43
http://hrcak.srce.hr/file/43
 
Source Croatica Chemica Acta; Vol.78 No.3; ISSN 0011-1643 (Print); ISSN 1334-417X (Online)
 
Language en
 
Rights info:eu-repo/semantics/openAccess
Parts of the contents of Croat. Chem. Acta (e. g. figures or tables) may be reproduced without prior permission, provided reference is made to their source.